MEASUREMENT OF THERMAL SYMMETRY OF THE HUMAN SPINE BY THE USE OF MEDICAL THERMOGRAPHY

The Aim of the study was to identify the temperature symmetry on physiologically healthy dorsal parts of torso. Because of problems with the correct focus on the central values of temperature on the dorsal side of the torso, the methodology of 3D body scanning was included. For the purposes of this study, two databases of 23 thermograms and 23 scans of dorsal trunks were created. The measured value of the average difference was 0.4°C. The 3D human body scanner was used for calculation of the length and size of spinal curvature in the sagittal plane. An important contribution of the study has been to establish a process for obtaining thermograms through the identification of focus area with 3D Body Scanner

THE BIOMECHANICAL MOVEMENTS FOR THE DISABLED BASED ON ART THERAPY

The purpose of the published work is to open up to new possibilities in the area of treatment processes within rehabilitation, physiotherapy and consequent movement re-education processes for people with disabilities, posttraumatic conditions, or degenerative disease. Art therapy presents a special kind of locomotive biomechanical algorithms focused on creative skills development, emotional development of physically or socially disturbed individuals. Art therapy is used for training and maintenance of motor and sensory functions, as a controlled and targeted activity during which the clients work with the artistic means of expression. The work proposes and describes a methodology, whose purpose is to follow and build on the classic rehabilitation of the physically disabled patient. To compare the effectiveness of the proposed methodology, the observations were made in two patient groups. The control group A and the experimental group B were included in the art therapy of the methodology of the ankle joint movement. After completing the art therapy, the experimental group B showed demonstrably better results in improving their plantar flexion by 8° on average compared to group A. The motivation and subsequent improvement of the patients’ agility is the best answer to questions of quality and contribution of using the Methodology of biomechanical movements using art therapy

APPLICATION OF THE ARTEC EVA SCANNER FOR ORTHOTICS IN PRACTICE

Tento článok sa týka spôsobov, ktoré sú k dispozícii na skeneri Artec Eva v oblasti protetiky a protetiky so zameraním na tvár a krk. Metodika je rozdelená do 5 základných skupín - príprava prostredia, skener, predmety, skenovanie a 3D modely. Praktická časť obsahuje 3D vybrané vybrané objekty, ktoré obsahujú ukážky iných druhov, konkrétne oblasti dosahu vlasov, brady a oblasti očí

DETERMINATION OF THE GEOMETRICAL AND VISCOELASTIC PROPERTIES OF SCAFFOLDS MADE BY ADDITIVE MANUFACTURING USING BIOPLOTTER

Additive Manufacturing (AM) is a name of a group of technologies that build 3D objects by adding layer-upon-layer of material. There are many technologies, including Rapid Prototyping (RP), Direct Digital Manufacturing (DDM), layered manufacturing and additive fabrication. Many types of materials can be used for AM technology. Biodegradable polymers such as polylactic acid (PLA) and polyhydroxybutyrate (PHB), are currently the subject of intensive research in the field of additive manufacturing and regenerative medicine. A number of biodegradable and bioresorbable materials, as well as scaffold designs, have been experimentally and clinically studied in many research facilities around the world. For effective using of bioprinting technologies in tissue and biomedical engineering, the knowledge of material and technological parameters in the process of printing is necessary. In this study the 3D printer Bioplotter EnvisionTEC (the printer with ability to print different materials from hydrogel to plastic materials) was used. Scaffolds for the purpose of the experiment were prepared via extrusion-based bioprinting. Experimental part of this study was focused on defining the influence of printing parameters and technological pre-processing of the material on quality and mechanical and geometrical properties of printed parts. Testing of printed samples showed high influence of pre-processing of material, mainly drying process, on mechanical and geometric quality of samples. Drying of material before printing process makes the material more stable and allows it to maintain defined material properties for a longer time than non-dried material. Time of heating of the material in printing cartridge has also high impact on material behaviour. Test results showed that if the time of heating of the material in the high temperature cartridge exceeds defined time limit, the material starts to degrade and is no more usable

METHODOLOGY OF THERMOGRAPHIC ATLAS OF THE HUMAN BODY

Thermography allows contactless measurement of surface temperature of objects, digital temperature display and graduate fields. Currently, this issue received increased attention since it is based on global efforts to give medical thermography in normal clinical practice. It is a noninvasive diagnostic method by which the disease can be diagnosed as early vascular system diseases, skin diseases, cancer, etc. The essence of research is to establish a reference sample thermograms using thermovision devices that convert thermal energy emitted. The reference sample of thermography was a statistically relevant number of healthy subjects, which was the basis for the creation of thermal atlas of the human body

THE EFFECT OF DOCETAXEL ON MOLECULAR MELTING PROFILE OF DNA EXTRACTED FROM HUMAN BREAST ADENOCARCINOMA MCF-7 CELLS

Aim: The aim of this study was to evaluate the genotoxic effect of docetaxel on the human adenocarcinoma MCF-7 cells to detect genetic variations and discover potential associations between the drug and the genotoxic damage of MCF-7 cells. Materials and Methods: High resolution melt analysis (HRM) of genomic DNA isolated from MCF-7 cells was used. Two primers of MDR1 gene were selected: 5´-TGGGGCTTTTAGTGTTGGAC-3´, 5´-TGTGGAGAGCTGGATAAAGTCA-3´. Results: The significant alterations in the melting temperature Tm of DNA treated with docetaxel in the concentrations of 1 μmol/l and 250 μmol/l were observed. Comparing G+C/A+T ratios the increase of the relative content of G, C was detected. Minor changes of the nucleotide content were observed when compared the sequences of DNA for the control and the docetaxel treated group. Conclusions: Using MDR1 primer pairs, our results confirmed that MCF-7 cells are susceptible to genomic DNA instability when exposed to docetaxel

THE EFFECT OF FOOTWEAR TO THE POSTURE

The aim of this study is to assess the effect of footwear on postural status of a group of volunteers representing the general population of female students. Based on the assumption that the elegant shoe with tapered toe and high heel does not provide adequate foot support, the study was designed to assess its direct link and impact to body segment alignment and resulting negative effect on posture. Repetition of this misalignment ensures the individual circumstances of posture. Due to the heel elevation, the weight is transferred to the distal part, resulting in a postural response of the entire musculoskeletal system to maintain balance. The group consisted of 30 women of the age 18–28 years with an average age of 22.7 years, height 167±0.3 cm and weight 57±0.9 kg. Anamnesis and aspection were performed to assess of the occurrence of shortened and weakened muscles and subsequently the patient's standing was examined. Two static methods were chosen for evaluation of the posture. The first one was the Silhouette Posture Analysis and the evaluation method by Jaros and Lomnicka. The result was the identification of typical muscle imbalance as the most common presumption of faulty posture, and it was confirmed that footwear affects a person's natural posture. Differences in the sensitivity of the two methods were also identified and studies are not only appropriate that but also evaluate differences in impact among subjects

CORELLATION THE BODY WEIGHT TO SELECTED STABILOMETRIC PARAMETERS

Mass is an important factor that has an influence on the stability of the human body. The hypothesis is the higher the mass, the higher the stability. The proportionality of the human body when considering body mass and physical height are expressed by the BMI (body mass index). Our goal was to verify a correlation between BMI and postural stability with the use of stabilometry tests on baropodometric platforms to confirm or refute the mentioned hypothesis. A total of 184 healthy subjects (73 females and 111 male) participated in the measurement and performed a bipedal test with their eyes open and closed, where the evaluated parameters were the confidence ellipse area and the length of the oscillation. After a statistical assessment of the measurements, we can confirm that having the eyes open or closed has an influence on the confidence ellipse area and the length of oscillation. The measured values show that the confidence ellipse area and the length of oscillation are not dependent on the BMI

Biocompatibility of the human mesenchymal stem cells with bovine bone tissue at the cellular level in vitro

The purpose of this study was to investigate biocompatibility of the human mesenchymal stem cells with bovine bone tissue at the cellular level in vitro. Phenotypic analysis of cells was made by flow cytometry. Cells were grown on the bone for 12 days. Metabolic activity of cells was assessed with the MTS assay. The growth data were used to calculate the population doubling times. The scanning electron microscopy was used to verify the attachment of cells on the bone surface. The results were analyzed by using ANOVA test. Immunophenotypic characteristics were positive for CD105, CD90, CD73, and negative for CD34, CD45. The growth curves of stem cells of the 1st and the 2nd passages for both media, with and without, bovine bone were constructed. The increase of approximately 60% of the doubling time for mesenchymal cells co-cultivated with bovine bone tissue was observed for both passages in comparison with the control.Our study confirmed that mesenchymal stem cells are able to adhere to the bovine bone, even not being modified with bone-targeting elements. The proliferation rate and metabolic activity of cells co-cultivated with bone decrease in comparison with the control. Better survival was observed for cells of the 1st passage